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By Peter Malcolm Wallis, Brian R. Hammond

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This simple in vitro assay system is rapid and sensitive compared with in vivo systems for the study of pathogenesis. Acknowledgements This work was supported by a research grant (70/288/85­ECD II) from the Indian Council of Medical Research.  62:216­ 226.  79:613­617.  Isoenzyme studies of Giardia lamblia isolated from symptomatic cases.  64:119­126.  54:1047­1056.  Plenum, New York.  56:849­882.  70:443­445.  86:1488­1495.  39:101­105.  17:1­47.  Path.  39:163­170.  Sci. 26:700­704.  36:295­307.

Gastroenterology 71:57­61.  33:1179­1183.  Microbiol. 45:65­74.  80:49­52.  Parasitologia 4:510­514.  12:851­853.  Science 92:416­417. A.  Cysts formed in vitro were both morphologically and immunologically similar to Giardia cysts formed in vivo and also were viable, as demonstrated by the uptake of fluorogenic dyes.  The inability of Giardia trophozoites to undergo encystation in vitro has raised questions as to whether host­ related factors, such as gut­associated microorganisms or nutritive factors and stimuli from the small intestine, might play a role in this process.

33:1179­1183.  Microbiol. 45:65­74.  80:49­52.  Parasitologia 4:510­514.  12:851­853.  Science 92:416­417. A.  Cysts formed in vitro were both morphologically and immunologically similar to Giardia cysts formed in vivo and also were viable, as demonstrated by the uptake of fluorogenic dyes.  The inability of Giardia trophozoites to undergo encystation in vitro has raised questions as to whether host­ related factors, such as gut­associated microorganisms or nutritive factors and stimuli from the small intestine, might play a role in this process.

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